ABSTRACT
We have previously shown that cycloheximide significantly inhibited apoptosis, and reduced ensuing cerebral infarction in a newborn rat model of cerebral hypoxiaischemia. This study was performed to determine the therapeutic window for cycloheximide therapy. Seven day-old newborn rat pups were subjected to 100 min of 8% oxygen following a unilateral carotid artery ligation, and cycloheximide was given at 0, 6, 12 and 24 hr after hypoxia-ischemia (HI). Apoptosis or necrosis was identified by performing flow cytometry with a combination of fluorescinated annexin V and propidium iodide, and the extent of cerebral infarction was evaluated with triphenyl tetrazolium chloride (TTC) at 48 hr and 72 hr after HI, respectively. With cycloheximide treatment at 0 hr after HI, both apoptotic and necrotic cells by flow cytometry were significantly reduced, only necrotic cells were significantly reduced at 6 and 12 hr, and no protective effect was seen if administration was delayed until 24 hr after HI compared to the HI control group. Infarct volume, measured by TTC, was significantly reduced by 92% and 61% when cycloheximide was given at 0 or 6 hr after HI respectively; however, there was an insignificant trend in infarct reduction if cycloheximide was administered 12 hr after HI, and no protective effect was observed when administration was delayed until 24 hr after HI. In summary, cycloheximide was neuroprotective when given within 6 hr after HI in the developing newborn rat brain.
Subject(s)
Rats , Humans , Animals , Rats, Sprague-Dawley , Protein Synthesis Inhibitors/therapeutic use , Oxygen/metabolism , Neuroprotective Agents/therapeutic use , Necrosis , Hypoxia-Ischemia, Brain/drug therapy , Hypoxia, Brain , Flow Cytometry , Cycloheximide/therapeutic use , Brain Ischemia , Apoptosis , Animals, NewbornABSTRACT
Se usó óvulos vaginales de Isodine (Isodine Ovulos) para tratar 33 mujeres eutiroideas no embarazadas portadoras de vulvovaginocervicitis a la dosis de un óvulo diario durante 14 días, con el fin de evaluar los posibles efectos en la función tiroidea. A nivel basal y post-tratamiento (día 15) se evaluaron los niveles sanguíneos de triyodotironina (T3), tiroxina (T4) y hormona estimulante del tiroides (TSH). El análisis de los datos preliminares y del rango de transformación de los valores de T3, T4 y TSH reveló que no se registraron cambios significativos en los niveles hormonales después del tratamiento. No se reportaron reacciones adversas al producto en estudio. Se concluye que los óvulos de Isodine no afectan en forma significativa la función tiroidea a la dosis administrada